Nickel-based alloys containing high amount of Cr are desirable materials for the production of critical components that operate under high temperature and severe corrosion conditions. The description of flow behaviors for these alloys is key to designing the hot formation process for achieving the excellent mechanical properties of components. This work aims to study the flow behaviors of Ni-38Cr-3.8Al alloy during thermal deformation. A series of isothermal compression experiments were carried out at temperatures of 1148 K, 1223 K, 1298 K, 1373 K, 1448 K and 1523 K, and strain rates of 0.01 s-1, 0.1 s-1, 1 s-1 and 10 s-1. Results show that the obtained true stress-strain curves exhibit the softening characteristics of dynamic recrystallization (DRX) and dynamic recovery (DRV). The flow stress decreases with temperature increasing and strain rate decreasing. To further quantitatively describe the flow behaviors, an improved segmented Arrhenius constitutive equation was developed according to the softening characteristics under different temperatures. In these equations, the variable coefficients, including activation energy Q and material constants (structure factor A, stress exponent n, stress parameters α), were fitted as the polynomial functions of true strain. Furthermore, a typical Arrhenius constitutive equation was also solved. The comparisons between experimental stress and predicted results obtained from the typical and improved constitutive equations were conducted, and the correlation coefficient (R) and average absolute relative error (AARE) were calculated as 0.932, 15.83%, and 0.9963, 5.61%, respectively. It suggests that the improved constitutive equation can adeptly describe the flow behaviors of Ni-38Cr-3.8Al alloy.

The objective of this study was to analyze the infection rate and drug resistance of Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) in the genitourinary tract of Chinese patients. From December 2018 to June 2019, vaginal secretion or urinary secretion of outpatients in our hospital were selected for culture and drug sensitivity analysis of Ureaplasma urealyticum and Mycoplasma hominis. In 4082 Chinese samples, 1567 Mycoplasma were detected, a detection rate of 38.39%, among which 1366 cases were UU single positive, accounting for 33.47%, 15 cases were MH single positive, accounting for 0.36%, 186 cases were UU and MH mixed positive, accounting for 4.56%. The most affected age groups were 21-30 years and 31-40 years, accounting for 19.09 and 15.05%, respectively. The results of drug sensitivity showed that doxycycline, minocycline, josamycin, clarithromycin, and roxithromycin were more sensitive to mycoplasma infection. The distribution of Ureaplasma urealyticum and Mycoplasma hominis in the human genitourinary system and their sensitivity to antibiotics is different for sex and age groups.

The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma.

Resumo Apresentamos o caso de uma paciente de 39 anos, com hipoxemia em risco de vida após a substituição da valva tricúspide devido à anomalia de Ebstein e cianose grave devido à estenose de valva bioprotética e comunicação interatrial. O manejo anestésico de um paciente com disfunção obstrutiva grave de prótese valvar pode ser um desafio. Os pacientes com anomalia de Ebstein também precisam de atenção especial para manter a pressão equalizada entre o átrio direito e o esquerdo. A ecocardiografia transesofágica e a saturação cerebral de oxigênio forneceram informações em tempo real nos cuidados perioperatórios.

Abstract We presented a 39-year-old female patient with life-threatening hypoxemia after tricuspid valve replacement because of Ebstein's anomaly. And the severe cyanosis is due to bioprosthetic valve stenosis and atrial septal defect. Anesthetic management of a patient with severe obstructive prosthetic valve dysfunction can be challenging. Similar considerations should be given to patients with Ebstein's anomaly to maintain the pressure equalized between the right and left atrial. Transesophageal echocardiography and cerebral oxygen saturation provided real time information in perioperative care.

Abstract Purpose: To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis. Methods: 90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology. Results: Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group. Conclusion: Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.

Candida haemulonii forms part of the non-albicans Candida species. The candidemia caused by C. haemulonii is extremely rare but fatal in neonates. We reported the first two neonates with C. haemulonii candidemia in China which were treated with fluconazole and reviewed two papers previously reported. Our report adds further awareness on C. haemulonii candidemia in critical neonates and points out the importance of an early diagnosis and treatment of this fatal infection.

La Candida haemulonii forma parte de la especie Candida no albicans. La candidemia por C. haemulonii es sumamente infrecuente, pero mortal, en los recién nacidos. Se informa sobre los dos primeros recién nacidos con candidemia por C. haemulonii en China tratados con fluconazol y se revisan dos artículos informados con anterioridad. Nuestro informe incrementa la sensibilización sobre la candidemia por C. haemulonii en recién nacidos críticos y resalta la importancia de un diagnóstico y un tratamiento tempranos de esta infección mortal.

Systemic lupus erythematosus (SLE) is a chronic, autoimmune disorder that affects nearly all organs and tissues. As knowledge about the mechanism of SLE has increased, some immunosuppressive agents have become routinely used in clinical care, and infections have become one of the direct causes of mortality in SLE patients. To identify the risk factors indicative of infection in SLE patients, a case control study of our hospital's medical records between 2011 and 2013 was performed. We reviewed the records of 117 SLE patients with infection and 61 SLE patients without infection. Changes in the levels of T cell subsets, immunoglobulin G (IgG), complement C3, complement C4, globulin, and anti-double-stranded DNA (anti-ds-DNA) were detected. CD4+ and CD4+/CD8+ T cell levels were significantly lower and CD8+ T cell levels were significantly greater in SLE patients with infection than in SLE patients without infection. Additionally, the concentrations of IgG in SLE patients with infection were significantly lower than those in SLE patients without infection. However, complement C3, complement C4, globulin, and anti-ds-DNA levels were not significantly different in SLE patients with and without infection. Therefore, clinical testing for T cell subsets and IgG is potentially useful for identifying the presence of infection in SLE patients and for distinguishing a lupus flare from an acute infection.

Indole-based receptors such as biindole, carbazole, and indolocarbazole are regarded as some of the most favorable anion receptors in molecular recognition. This is because indole groups possess N–H groups as hydrogen-bonding donors. The introduction of amide groups in the indole framework can induce strong binding properties and good water solubility. In this study, we designed and synthesized a series of N-(indol-3-ylglyoxylyl)benzylamine derivatives as novel and simple anion receptors. The receptors derived by aryl and aliphatic amines can selectively recognize F– based on a color change from colorless-to-yellow in DMSO. The receptors derived by hydrazine hydrate can recognize F–, AcO–, and H2PO4– by similar color changes in DMSO and can even enable the selective recognition of F– in a DMSO–H2O binary solution by the naked eye. Spectrographic data indicate that complexes are formed between receptors and anions through multiple hydrogen-bonding interactions in dual solutions.

Purpose: This cross-sectional study explored the prevalence of eating disorders (EDs) and possible associated factors (depressive symptoms, anxiety and BMI, etc) on EDs in female medical students in China. Methods: A total of 1,135 students in Wuhu, Anhui Province were sampled to participate in this survey. The survey collected sociodemographic data, depressive symptoms, anxiety and eating behaviors of students. Results: The students at risk for eating disorders were 2.17%. Eating disorder risk was more frequent in students with poor parent's relationship, students with poor relationship with parents, students with high body mass index, students who were excessive focus on slimming propaganda, and students whose relatives have a diet. Also, the mean scores for the psychological factors of depression and anxiety were higher in students with eating disorder risk. A multiple linear regression model was produced depicting that depression, anxiety, high level of BMI, focusing on slimming propaganda, and dieting status among relatives were risk factors of having an eating disorder risk. Conclusions: A significant fraction of female medical students in Anhui are at high risk of development of eating disorders. A comprehensive intervention focusing on family environment and emotion management should be designed to prevent occurrence of such disorders among medical students that would undoubtedly hamper the availability of dependable medical services in future.

Objetivo: Este estudio transversal exploró la prevalencia de los trastornos de la Alimentación (eds) y posibles factores asociados (síntomas depresivos, ansiedad y BMI, etc) sobre EDS en mujeres estudiantes de medicina en China. Métodos: un total de 1135 estudiantes en Wuhu, provincia de Anhui fueron seleccionadas para participar en esta encuesta.La encuesta recoge datos sociodemográficos, síntomas depresivos, ansiedad y conductas alimentarias de estudiantes. Resultados: los estudiantes en situación de riesgo para los trastornos alimentarios eran 2,17%.Trastorno alimenticio riesgo fue más frecuente en los estudiantes con la relación de los estudiantes con la pobre padre, mala relación con los padres, los alumnos con alto índice de masa corporal, los estudiantes que son la excesiva atención a la propaganda de adelgazamiento, y estudiantes cuyos familiares tienen una dieta.Además, la media de las puntuaciones para los factores psicológicos de la depresion y la ansiedad fueron mayores en los estudiantes con trastorno alimentario riesgo.Un modelo de regresion lineal multiple fue producido que muestran que la depresión, la ansiedad, el alto nivel de IMC, centrándose en la propaganda y la dieta de adelgazamiento, condición entre parientes eran factores de riesgo de tener un trastorno alimenticio y riesgo. Conclusiones: una importante fracción de mujeres estudiantes de medicina en Anhui están en alto riesgo de desarrollo de trastornos de la alimentación.Una intervención integral centrado en el entorno familiar y manejo de emociones deben ser diseñados para prevenir la ocurrencia de tales trastornos entre los estudiantes de medicina que, sin duda, obstaculizar la disponibilidad de servicios médicos de confianza en el futuro.

A novel BODIPY-based anion sensor 1 bearing di-tert-butyl phenol unit was designed and prepared. In the presence of three basic anions F-, AcO- or H2PO4-, 1 exhibited two novel partially overlapped red-shift absorption bands at 718 nm and 757 nm, respectively. The formation of allotropie structures, after deprotonation of phenol OH, is responsible for these two emerging bands. Fluorescence quenching was also observed due to intramolecular charge transfer (ICT) from phenolate to BODIPY core. As results revealed, the anion affinity mainly depended on the acidity of binding site OH and the basicity of the target anion. Moreover, two bulky tert-butyl groups cannot effectively exclude the approach of large anions to phenol OH, but can facilitate the formation of allotropic resonance structures.

South Asian monsoon occurs in most parts of the Indian subcontinent including Pakistan, India and Bangladesh during the period June to September of every year. These winds rich in moisture are forced to lift by the Himalayas causing extreme precipitation in different parts of the subcontinent, especially in Pakistan. The aim of this study is to explore the characteristics of atmospheric energy conversion and distribution during the heavy rainfall in Sindh, Pakistan for the period August-September 2011. The results show that when the energy value is greater than or equal to seven, the atmosphere jumps from stable to unstable. Under the influence of atmospheric circulations and terrain conditions, the kinetic energy is blocked by the mountains and is continuously converted into potential energy inside rainfall system. The peak phase of energy persisting for a considerable amount of time can lead to heavy rainfall. Moisture of this heavy rainfall mainly comes from the Arabian Sea and the Bay of Bengal.

El monzón del sudeste asiático se presenta todos los años de junio a septiembre en la mayor parte del subcontinente indio, lo cual incluye a Paquistán, la India y Bangladesh. Estos vientos ricos en humedad son forzados a elevarse por la cordillera del Himalaya, lo que ocasiona precipitación extrema en varias partes del subcontinente, especialmente en Paquistán. El objetivo de este estudio es analizar las características y distribución de la conversión de energía atmosférica durante la intensa precipitación registrada en Sindh, Paquistán, durante el periodo agosto-septiembre de 2011. Los resultados muestran que la atmósfera cambia de estable a inestable cuando el valor de la energía es igual o mayor a siete. Bajo la influencia de las circulaciones atmosféricas y las condiciones del terreno, la energía cinética es bloqueada por las montañas y convertida de manera continua en energía potencial dentro del sistema de precipitación. Cuando la fase de energía máxima persiste por un tiempo considerable, puede ocasionar precipitaciones intensas cuya humedad procede principalmente del Mar Arábico y la bahía de Bengala.

Objective: The aim of the study is to examine the distribution of integrated covariate and its association with blood pressure (BP) among children in Anhui province, China, and assess the predictive value of integrated covariate to children hypertension. Methods: A total of 2,828 subjects (1,588 male and 1,240 female) aged 7-17 years participated in this study. Height, weight, waistline, hipline and BP of all subjects were measured, obesity and overweight were defined by an international standard, specifying the measurement, the reference population, and the age and sex specific cut off points. High BP status was defined as systolic blood pressure (SBP) and/or diastolic blood pressure (DBP) > 95th percentile for age and gender. Results: Our results revealed that the prevalence of children hypertension was 11.03%, the SBP and DBP of obesity group were significantly higher than that of normal group. Anthropometric obesity indices such as body mass index (BMI) were positively correlated with SBP and DBP. Integrated covariate had a better performance than the single covariate in the receiver-operating characteristic (ROC) curve, the cut-off value; the sensitivity and the specificity of the integrated covariate were 0.112, 0.577, 0.683, respectively. Conclusion: Integrated covariate is a simple and effective anthropometric index to identify childhood hypertension.

Objetivo: El objetivo del estudio es examinar la distribución de las covariables integrado y su asociación con la presión arterial (PA) entre los niños en la provincia de Anhui, China, y evaluar el valor predictivo de covariables integrado a los niños la hipertensión. Métodos: Un total de 2.828 sujetos (1.588 macho y 1.240 hembra) de 7 a 17 años participaron en este estudio. Altura, peso, cintura, hipline y BP de todos los sujetos fueron medidos, la obesidad y el sobrepeso se han definido por una norma internacional, especificando la medición, la población de referencia, y la edad y sexo los puntos de corte. La condición de alta presión se define como la presión arterial sistólica (PAS) y/o presión arterial diastólica (PAD) > percentil para edad y sexo. Resultados: Nuestros resultados revelan que la prevalencia de niños la hipertensión fue 11,03%, el SBP y DBP de obesidad grupo fueron significativamente más alta que la de grupo normal. Los índices de obesidad, tales como el índice de masa corporal (IMC) se correlacionaron positivamente con SBP y DBP. Integrated covariable tuvo un mejor rendimiento que la covariable en la curva de características operativas del receptor (ROC), el valor de corte, la sensibilidad y la especificidad de las covariables fueron integrados 0,112, 0,577,0,683, respectivamente. Conclusión: Integrado covariable es un simple y eficaz para identificar a la niñez índice antropométrico hipertensión.

As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic acid from multiple copies integrant was about 4 times higher than that from single copy (1017.13 μg/ml VS. 198.89 μg/ml). And when induced by maltose the production of pimelic acid was about 2 times of that under non-induction conditions (2360.73 μg/ml VS. 991.59 μg/ml). Thus, these results demonstrated that the production of pimelic acid was improved obviously through reconstructed B. subtilis. It also suggested that our expression system provided a convenient source of pimelic acid that would potentially lower the cost of production of biotin from engineered B. subtilis.

Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They are flanked by 5 bp direct repeats (ACCAT, CTTTC). In Boty-II-141, two LTRs at both ends, and a partial internal pol gene encoding a protein with a DDE integrase domain were identified. In Boty-II-95, a right LTR and a partial internal pol gene encoding a protein with no conserved domains were identified. Boty-II uses a self-priming mechanism to initiate synthesis of reverse transcripts. The sequence of the presumed primer binding site for first-strand reverse transcription is 5’-TTGTACCAT-3’. The polypurine-rich sequence for plus-strand DNA synthesis is 5’-GCCTTGAGCGGGGGGTAC-3’. Fourteen Boty-II LTRs that contain 125-158 bp nucleotides and share 69.1 ~ 100% identities with the short inverted terminal repeats of 5 bp (TGTCA…TGACA) were discovered. Analysis of structural features and phylogeny revealed that Boty-II is a novel LTR retrotransposon. It could potentially be used as a novel molecular marker for the investigation of genetic variation in B. cinerea.

The plant hormone abscisic acid has huge economic potential and can be applied in agriculture and forestry for it is considered to be involved in plant resistance to stresses such as cold, heat, salinity, drought, pathogens and wounding. Now overproducing strains of Botrytis cinerea are used for biotechnological production of abscisic acid. An LTR retrotransposon, Boty-aba, and a solo LTR were identified by in silico genomic sequence analysis, and both were detected within the abscisic acid gene cluster in B. cinerea B05.10, but not in B. cinerea SAS56. Boty-aba contains a pair of LTRs and two internal genes. The LTRs and the first gene have features characteristic of Ty3/gypsy LTR retrotransposons. The second gene is a novel gene, named brtn, which encodes for a protein (named BRTN) without putative conserved domains. The impressive divergence in structure of the abscisic acid gene clusters putatively gives new clues to investigate the divergence in the abscisic acid production yields of different B. cinerea strains.